Optimization of extraction conditions for polyphenols from the stem bark of Funtumia elastica (Funtum) utilizing response surface methodology [version 1; peer review: awaiting peer review]
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Date
2021
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PubMed Central
Abstract
Background: The recovery of phenolic compounds is seen as an
arduous task because phenolic compounds are available as free
aglycones, as sugar or ester conjugates, or as polymers with several
monomeric components. Furthermore, phenolic compounds do not
disperse evenly and may be connected to cell walls, carbohydrates, or
proteins. This study looks at the optimization of factors that affect the
efficiency for the extraction of phenolic compounds from the stembark
of Funtumia elastica.
Methods: Five independent variables (solvent concentration, time, the
temperature, solid-liquid ratio, and pH) of the extraction process were
selected. Single factor analysis as well as the response surface method
was used to evaluate the impact of the selected factors on the total
phenolic content. The effect of the extraction factors on the phenolic
content was statistically significant (p <0.05). For the response surface
method, a five/factor, five/level central composite design used, and a
fitted second-order polynomial regression model equation was used
to show how the extraction parameters affected the total phenolic
recovery.
Results: The predicted value (R² of 0.5917) agreed with the adjusted
value (R² of 0.7707). The residuals for response predictions were less
than 5%. The optimal factors for the extraction were ethanol
concentration of 75.99% v/v, extraction time of 193.86 minutes,
temperature of 63.66°C, pH of 5.62, and solid-liquid ratio of 1:21.12
g/mL. Actual overall content of the phenolic compounds was validated
at 82.83 ± 3.335 mg gallic acid equivalent (GAE) /g weight of extract,
which agreed with the predicted response of 89.467 mg GAE/g of the
dried extract under the optimal factors.
Conclusions: The rich phenolic content of stem-bark of Funtumia
elastica points to its potential as a functional medicinal product to
alleviate diseases caused by oxidative stress such as asthma,
Description
This article is published by Pubmed Central and is also available at https://doi.org/10.12688/aasopenres.13284.1
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Citation
Fadjare Frempong T, Owusu Boadi N, Badu M. Optimization of extraction conditions for polyphenols from the stem bark of Funtumia elastica (Funtum) utilizing response surface methodology. AAS Open Res. 2021 Oct 25;4:46. doi: 10.12688/aasopenres.13284.2. PMID: 34632271; PMCID: PMC8479850.