Genetic Diversity of PCR-Positive, Culture-Negative and Culture-Positive Mycobacterium ulcerans Isolated from Buruli Ulcer Patients in Ghana

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dc.contributor.authorWilliamson, Heather
dc.contributor.authorPhillips, Richard Odame
dc.contributor.authorSarfo, Fred Stephen
dc.contributor.authorWansbrough-Jones, Mark
dc.contributor.authorSmall, Pamela
dc.date.accessioned2020-01-09T16:35:56Z
dc.date.accessioned2023-04-19T01:42:04Z
dc.date.available2020-01-09T16:35:56Z
dc.date.available2023-04-19T01:42:04Z
dc.date.issued2014-02-10
dc.descriptionAn article published by PLOS ONE and available at doi:10.1371/journal.pone.0088007en_US
dc.description.abstractCulture of Mycobacterium ulcerans from Buruli ulcer patients has very low sensitivity. Thus confirmation of M. ulcerans infection is primarily based on PCR directed against IS2404. In this study we compare the genotypes obtained by variable number of tandem repeat analysis of DNA from IS2404-PCR positive cultures with that obtained from IS2404 positive, culture-negative tissue. A significantly greater genetic heterogeneity was found among culture-negative samples compared with that found in cultured strains but a single genotype is over-represented in both sample sets. This study provides evidence that both the focal location of bacteria in a lesion as well as differences in the ability to culture a particular genotype may underlie the low sensitivity of culture. Though preliminary, data from this work also suggests that mycobacteria previously associated with fish disease (M. pseudoshottsii) may be pathogenic for humans.en_US
dc.description.sponsorshipKNUSTen_US
dc.identifier.citationPLoS ONE 9(2): e88007. doi:10.1371/journal.pone.0088007en_US
dc.identifier.urihttps://ir.knust.edu.gh/handle/123456789/11879
dc.language.isoenen_US
dc.publisherPLOS Oneen_US
dc.titleGenetic Diversity of PCR-Positive, Culture-Negative and Culture-Positive Mycobacterium ulcerans Isolated from Buruli Ulcer Patients in Ghanaen_US
dc.typeArticleen_US
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