Molecular characterization and antibiotic susceptibility of vibrio cholerae isolates in Southern Ghana

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This study was carried out to determine the presence or absence of virulence genes in Vibrio cholera isolates in Ghana, and also to determine susceptibility of the isolates to commonly prescribed antibiotics for the treatment of cholera in Ghana. Forty (40) Vibrio cholera isolates obtained from the National Public Health and Reference Laboratory, Korle-Bu and Komfo-Anokye Teaching Hospital were used for the analysis. All 40 isolates were susceptible to tetracycline and ciprofloxacin but resistance was demonstrated in doxycycline, ampicillin cotrimoxazole, and erythromycin. Thirty-nine of the isolates showed presence of genes coding for virulence (toxin coregulated pillus and cholera toxin gene) whiles SXT element was also present in 39 Vibrio cholera isolates. Pulse field gel electrophoresis on the isolates showed clusters similar to strains from DR Congo, Togo, Cameroun and Ivory Coast.
A thesis submitted to the Department of Clinical Microbiology, School of Medical Sciences Kwame Nkrumah University of Science and Technology in partial fulfillment of the requirement for the award of the degree of master of science (M.Sc.) in Clinical Microbiology,