Microscopy for Acid Fast Bacilli: A Useful but Neglected Tool in Routine Laboratory Diagnosis of Buruli Ulcer

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dc.contributor.authorPhillips, Richard Odame
dc.contributor.authorFrimpong, Michael
dc.contributor.authorSarpong-Duah, Mabel
dc.contributor.authorBeissner, M
dc.contributor.authorSarfo, Fred Stephen
dc.contributor.authoret. al
dc.date.accessioned2020-01-13T11:04:31Z
dc.date.accessioned2023-04-19T01:46:08Z
dc.date.available2020-01-13T11:04:31Z
dc.date.available2023-04-19T01:46:08Z
dc.date.issued2015-05-18
dc.descriptionAn article published by Journal of Tropical Diseases and available at doi:10.4172/2329891X.1000158en_US
dc.description.abstractBackground: Laboratory diagnosis of Buruli ulcer disease has become vital with the introduction of antibiotic treatment. Polymerase chain reaction (PCR) for the IS2404 repeat sequence of Mycobacterium ulcerans is the gold standard for laboratory diagnosis. This is expensive and only carried out in reference laboratories in endemic countries in Africa. In order to improve the efficiency of diagnosis at the point of care and reduce the total cost of patient management, we decided to evaluate Ziehl-Neelsen (ZN) staining for acid-fast bacilli (AFB) as an inexpensive diagnostic tool. Methods: Two smears directly prepared at the point of care were examined under oil-immersion microscopy after ZN staining for AFB and compared the results to PCR samples from the same patients. Results: Good quality smears were obtained from all subjects and our results showed that when a second smear was added the sensitivity of microscopy for AFB was increased from 52 to 55% for FNA samples and from 51 to 57 % for swabs. Conclusion: If PCR were to be omitted in all patients with suspected Buruli ulcer disease when AFB were detected it would result in a considerable saving.en_US
dc.description.sponsorshipKNUSTen_US
dc.identifier.citationJ Trop Dis 3: 158. doi:10.4172/2329891X.1000158en_US
dc.identifier.urihttps://ir.knust.edu.gh/handle/123456789/11914
dc.language.isoenen_US
dc.publisherJournal of Tropical Diseasesen_US
dc.subjectMycobacterium ulceransen_US
dc.subjectBuruli ulceren_US
dc.subjectZiehl-Neelsen stainingen_US
dc.subjectPolymerase chain reactionen_US
dc.titleMicroscopy for Acid Fast Bacilli: A Useful but Neglected Tool in Routine Laboratory Diagnosis of Buruli Ulceren_US
dc.typeArticleen_US
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