Survey and characterisation of Glucose-6-Phosphate Dehydrogenase variants and haemoglobin types in Ghana

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Venous blood samples obtained from 1,680 healthy Ghanaians belonging to some of the major ethnic groups in Ghana were analysed using the methaemoglobin reduction test, vertical polymerylamide gel electrophoresis at PH 8.6 and spectrophotometric methods: and for haemaglobin types, using vertical polyacrylamide gel electrophoresis at PH 8.6, paper electrophoresis at PH 8.6 agar gel electrophoresis at PH 8.6, paper electrophoresis at PH 8.6, agar gel electrophoresis at PH 6.4 and polypeptide chain analysis on cellulose acetate strips. The overall frequency of the of the G-6-PD deficiency was found to be 13.6% with the highest frequency in the Ewe Group, located in the south-eastern part of Ghana and the lowest frequency in the Mamprusi group, located in the north-eastern part of Ghana. The frequency of the enzyme deficiency declines as one moves westwards and inland from the geographical location of the Ewe Group.
A thesis presented for the degree of Master of Science, University of Science and Technology, Kumasi, Ghana, 1973