Comparison of an UV-Spectrophotometric method and HPLC method for the assay of Co-trimoxazole preparations

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Bacterial infections are prevalent in the country. Co-trimoxazole an antibacterial agent is effective against a large number of bacteria. This agent due to the low cost appears on the local market from various countries. It is important that a rapid, simple, reproducible and accurate method is designed for its analysis. Modem trends in drug analysis prefer the use of fast, reproducible, simple and convenient methods. An HPLC method for the quantification of cotrirnoxazole is described. The method involved dissolution of an amount of the powdered drug sample equivalent to 100mg sulphai.nethoxazole and 20mg trimethoprim in l00ml of ethanol and further dilution of the solution such that the final concentration of suiphainethoxazole and trimethoprirn was .40μg/ml and 8μg/ml respectively. Chromatographic separation was achieved on a Hypersil C-18 reversed phased column. Column cluate was monitored at 254nm using a UV detector. The isocratic form of elution was employed at a flow rate of 0.8mllminute and the column eluate monitored at a wavelength of 254nna and 0.5 AUFS. Chromatography was carried out at ambient temperature. Total chromatography time was approximately 7 minutes. Quantification was achieved using the method of internal calibration with procaine hydrochloride serving as internal standard. The method was used to analyze some brands of cotrirnoxazole on the local market and the results obtained compared statistically to an existing UV method. it was generally observed that the selected brands conformed to the BP specified quantities of active principle
A thesis submitted to the Board of Postgraduate Studies, Kwame Nkrumah University of Science and Technology, Kumasi, in partial fulfilment for the requirements for the award of the degree of Master of Science in Pharmaceutical Analysis and Quality Control, 2000