Molecular characterization of mycobacterial species isolated from tuberculosis lesions seen in bovine carcasses at post mortem: a case study of three abattoirs in the Accra Region of Ghana.

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dc.contributor.authorAtiadeve, Samuel Kumah
dc.date.accessioned2011-07-18T15:43:06Z
dc.date.accessioned2023-04-21T00:04:59Z
dc.date.available2011-07-18T15:43:06Z
dc.date.available2023-04-21T00:04:59Z
dc.date.issuedOctober 2010
dc.descriptionA Thesis submitted to the Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology in partial fulfillment of the requirements for the degree of Master of Scienceen_US
dc.description.abstractTuberculosis in cattle poses a serious public health threat and yet there is very little information on its extent in Ghana. A study to screen and characterize suspicious carcasses at post-mortem in three selected abattoirs in Accra was conducted. A total of 2,886 cattle slaughtered between June and October, 2009 were examined at post-mortem. One hundred and fifty five (155) suspicious tissue samples were taken from 145 bovines and subjected to Ziehl-Neelsen (ZN) microscopy and subsequently cultured on Löwenstein-Jensen (L-J) media. Isolates were harvested and subjected to ZN microscopy and then screened with the Capilia TB-Neo test and also characterized by a 13-probe Multiplex Ligation-dependent Probe Amplification assay (MLPA). The results revealed an apparent lesion prevalence of 5.0%. ZN microscopy indicated that 32.3% (50/155) of tissues were acid fast. Culture on L-J yielded 85.8% (133/155) isolates. One hundred and twenty-nine out of one hundred and fifty-five (129/155 or 97%) of these were acid fast by ZN microscopy. The Capilia TB-Neo test was performed on 93 of the isolates. Capilia TB-Neo test identified 11.8% (11/ 93) as members of the Mycobacterium tuberculosis Complex (MTBC) and 88.2% (82/93) as Non tuberculous mycobacteria (NTM). A further characterization of isolates using MLPA revealed that 3.8% (5/130) were M. africanum and 6.2% (8/130) were M. bovis. Surprisingly, 38.5 % (50/130) were classified as MTB (not M. bovis or M. africanum). Mixed infection was also identified in 42.9% (3/7) bovines. A comparison of MLPA and Capilia TB-Neo tests using the latter as a “Gold Standard” revealed that MLPA has a sensitivity and specificity of 72.7% and 39.7% respectively. The positive predictive value (PPV) and negative predictive value (NPV) were 16.3% and 90.0% respectively. The data obtained indicates that contamination of beef by TB is prevalent and therefore could pose a serious health threat. More stringent management and control methods should be put in place to improve beef quality.en_US
dc.identifier.urihttps://ir.knust.edu.gh/handle/123456789/419
dc.language.isoenen_US
dc.titleMolecular characterization of mycobacterial species isolated from tuberculosis lesions seen in bovine carcasses at post mortem: a case study of three abattoirs in the Accra Region of Ghana.en_US
dc.typeArticleen_US
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