Rapid detection of Mycobacterium ulcerans with isothermal recombinase polymerase amplification assay

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dc.contributor.authorPhillips, Richard Odame
dc.contributor.authorFrimpong, Michael
dc.contributor.authorAhor, Hubert Senanu
dc.contributor.authorWahed, Ahmed Abd El
dc.contributor.authorAgbavor, Bernadette
dc.contributor.authoret. al
dc.date.accessioned2020-01-13T12:19:45Z
dc.date.accessioned2023-04-19T01:47:16Z
dc.date.available2020-01-13T12:19:45Z
dc.date.available2023-04-19T01:47:16Z
dc.date.issued2019-02-01
dc.descriptionAn article published by PLOS Neglected Tropical Diseases and available at doi.org/10.1371/journal.pntd.0007155en_US
dc.description.abstractAccess to an accurate diagnostic test for Buruli ulcer (BU) is a research priority according to the World Health Organization. Nucleic acid amplification of insertion sequence IS2404 by polymerase chain reaction (PCR) is the most sensitive and specific method to detect Mycobacterium ulcerans (M. ulcerans), the causative agent of BU. However, PCR is not always available in endemic communities in Africa due to its cost and technological sophistication. Isothermal DNA amplification systems such as the recombinase polymerase amplification (RPA) have emerged as a molecular diagnostic tool with similar accuracy to PCR but having the advantage of amplifying a template DNA at a constant lower temperature in a shorter time. The aim of this study was to develop RPA for the detection of M. ulcerans and evaluate its use in Buruli ulcer disease.en_US
dc.description.sponsorshipKNUSTen_US
dc.identifier.citationPLoS Negl Trop Dis 13(2): e0007155. https://doi.org/10.1371/journal.pntd.0007155en_US
dc.identifier.urihttps://ir.knust.edu.gh/handle/123456789/11919
dc.language.isoenen_US
dc.publisherPLOS Neglected Tropical Diseasesen_US
dc.titleRapid detection of Mycobacterium ulcerans with isothermal recombinase polymerase amplification assayen_US
dc.typeArticleen_US
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