Method Development for the Quantification of Piperaquine Phosphate and Dihydroartemisinin in Combination Tablets

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dc.contributor.authorKissi, Freda Dansoah
dc.date.accessioned2011-07-14T16:28:53Z
dc.date.accessioned2023-04-20T12:20:10Z
dc.date.available2011-07-14T16:28:53Z
dc.date.available2023-04-20T12:20:10Z
dc.date.issued2010-07-14
dc.descriptionA thesis submitted in partial fulfilment of the requirements for the degree of MASTER OF SCIENCE in PHARMACEUTICAL ANALYSIS AND QUALITY CONTROL, 2010en_US
dc.description.abstractThis research developed a spectrometric method of analyzing Piperaquine phosphate and Dihydroartemisinin in combination tablets. Piperaquine phosphate in the tablets was analyzed by extracting with 0.001M HCl solution and taking its absorbance at 349nm. The LOD was 5.56 x 10 -5 %w/v and the LOQ was 1.68 x 10 -4 %w/v. HPLC conditions were ODS column with a mobile phase of acetonitrile and water with 0.1%v/v Trifluoroacetic acid (TFA), (40:60) at a flow rate of 1.0ml/min at a wavelength of 349nm and an injection volume of 20μl. The retention time was 3.2 minutes. The LOD was 4.9 x 10 -5 %w/v and the LOQ was 1.48 x 10 -4 %w/v. Dihydroartemisinin in the tablets was analysed by sonicating the tablet powder in diethyl ether for some time, filtering and evaporating the diethyl ether off. The residue was dissolved in methanol and reacted with conc HCl for 30 minutes at 30OC and the absorbance taken at 254nm. The LOD was 1.09 x 10 -4 %w/v and the LOQ was 3.31 x 10 -3 %w/v. HPLC conditions were ODS column with a mobile phase of acetonitrile and water with 0.1%v/v TFA (90:10) at a flow rate of 1.0ml/min at a wavelength of 220nm and an injection volume of 20μl.The retention time was 3.8 minutes. The LOD was 0.013 %w/v and the LOQ was 0.040 %w/v.en_US
dc.description.sponsorshipKNUSTen_US
dc.identifier.urihttps://ir.knust.edu.gh/handle/123456789/318
dc.language.isoenen_US
dc.titleMethod Development for the Quantification of Piperaquine Phosphate and Dihydroartemisinin in Combination Tabletsen_US
dc.typeArticleen_US
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