In vitro antimicrobial, antioxidant, and anti-helminthic activities and GC-MS analysis of fractions of the leaves of Aspilia silphioides
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Date
2024-08
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Journal of Pharmacognosy and Phytochemistry
Abstract
The leaves of Aspilia silphioides are effective for wound healing, stomach aches, headaches, worm infestations, and birth control. The purpose of this study was to determine the phytochemical components of the bulked fractions of A. silphioides and evaluate the antimicrobial, anti-helminthic, and antioxidant activities of various solvent fractions. Standard methods were employed for the phytochemical screening. The methanol extract was fractionated and bulked to obtain bulked fractions A, B, C and D. The bulked fractions contained phytoconstituents including tannins, flavonoids, phenolics, steroids, polyphenols, phytosterols, and terpenoids. GC-MS analysis of the fractions revealed various compounds including 9-octadecenamide, O-methyl- (+)-a-tocopherol, and a-Amyrin as being present in the bulked fractions. The IC50 of the fractions B, C, D and the reference drug with regards to the DPPH scavenging activity were 15.47±0.61 μg/mL, 28.16 ±0.61 μg/mL, 19.62 ±0.61 μg/mL, and 40.29±0.62 μg/mL respectively. At 20 μg/mL, the paralysis and death time for both fraction C and Mebendazole drug were 19.45±0.15 min, 31.60±18 min, and 52.06±2.89min, 82.03±47.37min respectively. The minimum inhibitory concentrations of fractions A, C, and D against the tested organisms were 25±0.01-50±0.01 mg/mL, 6.25±0.02-25±0.01 mg/mL, and 12.5±0.01-25±0.01 mg/mL respectively. The minimum bactericidal concentrations of fractions A, C, and D against the tested organisms were 25±0.01-50±0.01 mg/mL, 6.25±0.01-25±0.01 mg/mL, and 12.5±0.01-50±0.02 mg/mL respectively. The bulked fractions of the methanol extracts of Aspilia silphioides possess antioxidant, anti-helminthic, and antimicrobial activities.
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This article is published by Journal of Pharmacognosy and Phytochemistry 2024 and is also available at https://doi.org/10.22271/phyto.2024.v13.i5c.15071