Simple, Rapid Mycobacterium ulcerans Disease Diagnosis from Clinical Samples by Fluorescence of Mycolactone on Thin Layer Chromatography

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dc.contributor.authorWadagni, Anita
dc.contributor.authorFrimpong, Michael
dc.contributor.authorPhanzu, Delphin Mavinga
dc.contributor.authorPhillips, Richard O.
dc.contributor.authorAblordey, Anthony
dc.date.accessioned2020-01-09T15:17:00Z
dc.date.accessioned2023-04-19T01:41:28Z
dc.date.available2020-01-09T15:17:00Z
dc.date.available2023-04-19T01:41:28Z
dc.date.issued2015-11-19
dc.descriptionAn article published by PLOS Neglected Tropical Diseases and is available at DOI:10.1371/journal.pntd.0004247en_US
dc.description.abstractMycobacterium ulcerans infection, known as Buruli ulcer, is a disease of the skin and subcutaneous tissues which is an important but neglected tropical disease with its major impact in rural parts of West and Central Africa where facilities for diagnosis and management are poorly developed. We evaluated fluorescent thin layer chromatography (f-TLC) for detection of mycolactone in the laboratory using samples from patients with Buruli ulcer and patients with similar lesions that gave a negative result on PCR for the IS2404 repeat sequence of M. ulcerans Methodology/Principal findings Mycolactone and DNA extracts from fine needle aspiration (FNA), swabs and biopsy specimen were used to determine the sensitivity and specificity of f-TLC when compared with PCR for the IS2404. For 71 IS2404 PCR positive and 28 PCR negative samples the sensitivity was 73.2% and specificity of 85.7%for f-TLC. The sensitivity was similar for swabs (73%), FNAs (75%) and biopsies (70%).en_US
dc.description.sponsorshipKNUSTen_US
dc.identifier.citationPLoS Negl Trop Dis 9(11): e0004247. doi:10.1371/journal. pntd.0004247en_US
dc.identifier.urihttps://ir.knust.edu.gh/handle/123456789/11875
dc.language.isoenen_US
dc.publisherPLOS Neglected Tropical Diseasesen_US
dc.titleSimple, Rapid Mycobacterium ulcerans Disease Diagnosis from Clinical Samples by Fluorescence of Mycolactone on Thin Layer Chromatographyen_US
dc.typeArticleen_US
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