Loop-Mediated Isothermal Amplification for Laboratory Confirmation of Buruli Ulcer Disease—Towards a Point-of-Care Test

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Date
2015-11-13
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PLOS Neglected Tropical Diseases
Abstract
As the major burden of Buruli ulcer disease (BUD) occurs in remote rural areas, development of point-of-care (POC) tests is considered a research priority to bring diagnostic services closer to the patients. Loop-mediated isothermal amplification (LAMP), a simple, robust and cost-effective technology, has been selected as a promising POC test candidate. Three BUD-specific LAMP assays are available to date, but various technical challenges still hamper decentralized application. To overcome the requirement of cold-chains for transport and storage of reagents, the aim of this study was to establish a dry-reagentbased LAMP assay (DRB-LAMP) employing lyophilized reagents.
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An article published by PLOS Neglected Tropical Diseases
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PLoS Negl Trop Dis 9(11): e0004219. doi:10.1371/journal.pntd.0004219
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https://ir.knust.edu.gh/handle/123456789/11864
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