Browsing by Author "Buah, Patrick"

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    In vitro anthelminthic and anti-inflammatory activities, and GC-MS analysis of methanol and acetone extracts of Mareya micrantha leaves
    (Journal of Pharmacognosy and Phytochemistry, 2024-03) Acheampong, Akwasi; Quartey, Emmanuel; Acquaye, Maud Adoley; Naazo, Abdulai A; Baah, Kennedy Ameyaw; Amankwaa, Lydia Tima; Buah, Patrick; Frimpong, Silas Ofori; 0000-0003-2481-1126
    Mareya micrantha, a medicinal plant, is used to treat pains, wounds, worm infestations and gastrointestinal disorders. The aim of this research was to investigate the anthelmintic and anti-inflammatory properties of the methanol and acetone extracts of Mareya micrantha leaves. Phytochemical screening was performed using standard methods with GC-MS used for the identification of the phytochemicals. Egg albumen denaturation was used for the determination of the anti-inflammatory (in vitro) activities of the extracts. Anthelmintic activity (in vitro) of the extracts was investigated against Millsonia ghanensis. Phytochemical investigation revealed the presence of phenols, terpenoids, polyphenols, flavonoids, tannins, steroids, saponins, and glycosides. Twenty phytochemicals, most of which have known bioactivities, were identified for each extract with five being common to them, and they are n-hexadecanoic acid, Tributyl acetyl citrate, hexadecanoic acid methyl ester, 3, 7, 11, 15-tetramethyl-2-hexadecen-1-ol, and 2, 2, 4-trimethyl-3-(3, 8, 12, 16-tetramethyl-heptadeca-3, 7, 11, 15-tetraethyl)-cyclohexanol. The extracts had anti-inflammatory activity. The anthelmintic activity of the extracts was significantly higher than mebendazole-treated helminths. The outcome of this study points to the fact that Mareya micrantha could be exploited as a source of potential drug candidates against helminthic and microbial infection as well as inflammation and oxidative stress.
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    In vitro antimicrobial, antioxidant, and anti-helminthic activities and GC-MS analysis of fractions of the leaves of Aspilia silphioides
    (Journal of Pharmacognosy and Phytochemistry, 2024-08) Acheampong, Akwasi; Buah, Patrick; Oti-Boakye, Adolf; 0000-0003-2481-1126
    The leaves of Aspilia silphioides are effective for wound healing, stomach aches, headaches, worm infestations, and birth control. The purpose of this study was to determine the phytochemical components of the bulked fractions of A. silphioides and evaluate the antimicrobial, anti-helminthic, and antioxidant activities of various solvent fractions. Standard methods were employed for the phytochemical screening. The methanol extract was fractionated and bulked to obtain bulked fractions A, B, C and D. The bulked fractions contained phytoconstituents including tannins, flavonoids, phenolics, steroids, polyphenols, phytosterols, and terpenoids. GC-MS analysis of the fractions revealed various compounds including 9-octadecenamide, O-methyl- (+)-a-tocopherol, and a-Amyrin as being present in the bulked fractions. The IC50 of the fractions B, C, D and the reference drug with regards to the DPPH scavenging activity were 15.47±0.61 μg/mL, 28.16 ±0.61 μg/mL, 19.62 ±0.61 μg/mL, and 40.29±0.62 μg/mL respectively. At 20 μg/mL, the paralysis and death time for both fraction C and Mebendazole drug were 19.45±0.15 min, 31.60±18 min, and 52.06±2.89min, 82.03±47.37min respectively. The minimum inhibitory concentrations of fractions A, C, and D against the tested organisms were 25±0.01-50±0.01 mg/mL, 6.25±0.02-25±0.01 mg/mL, and 12.5±0.01-25±0.01 mg/mL respectively. The minimum bactericidal concentrations of fractions A, C, and D against the tested organisms were 25±0.01-50±0.01 mg/mL, 6.25±0.01-25±0.01 mg/mL, and 12.5±0.01-50±0.02 mg/mL respectively. The bulked fractions of the methanol extracts of Aspilia silphioides possess antioxidant, anti-helminthic, and antimicrobial activities.