Extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in local and imported poultry meat in Ghana
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Date
2018
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Abstract
Antibiotic use in animal husbandry has raised concerns on the spread of resistant bacteria. Currently animal
products are traded globally with unprecedented ease, which has been challenging the control of antimicrobial
resistance. This study aims to detect and characterize extended-spectrum beta-lactamase (ESBL)-producing
Escherichia coli and Klebsiella pneumoniae from imported and locally produced poultry products sold in Ghana.
Local and imported chicken meat was collected from 94 stores and markets throughout Kumasi (Ghana) and
cultured on selective ESBL screening agar. Phenotypic ESBL-producing E. coli and K. pneumoniae isolates were
confirmed by combined disc test and further characterized by antibiotic susceptibility testing, amplification of
the blaCTX-M, blaTEM and blaSHV genes as well as multilocus sequence typing (MLST) and linked to the country
of origin.
Out of 200 meat samples, 71 (36%) samples revealed 81 ESBL-producing isolates (46 E. coli and 35 K.
pneumoniae), with 44% (30/68) of local poultry and 31% (41/132) of imported products being contaminated.
Most ESBL-producing isolates harboured the blaCTX-M-15 gene (61/81, 75%) and the dominant Sequence Types
(ST) were ST2570 (7/35, 20%) among K. pneumoniae and ST10 (5/46, 11%) among E. coli.
High numbers of ESBL-producing bacteria, particularly on local but also imported poultry meat, represent a
potential source for human colonization and infection as well as spread within the community. Surveillance
along the poultry production-food-consumer chain would be a valuable tool to identify sources of emerging
multidrug resistant pathogens in Ghana
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This article is published by Elsevier and is also available at www.elsevier.com/locate/vetmic
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Veterinary Microbiology 217 (2018) 7-12